p akt ser473  (Proteintech)

Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: p-AKT Ser473 , Proteintech , 66444-1-Ig.

Techniques: Cell-Signaling

Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: α-Tubulin , Proteintech , 11224-1-AP.

Techniques: Cell-Signaling

Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: GAPDH , Proteintech , 60004-1-Ig.

Techniques: Cell-Signaling

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: AMOA promotes osteoclast and inflammatory remodeling in periodontal area. (a) Representative images of immunohistochemical staining for CTSK, TNF‐α, IL‐1β. CTSK staining at the periodontal ligament‑alveolar bone interface. Scale bar: 100 µm(CTSK), 20 µm(TNF‐α), and 20 µm(IL‐1β). (b) Statistics of numbers of CTSK positive cells in the alveolar bone and percentage of TNF‐α, IL‐1β positive cells per field in the periodontal ligament of the first molar. Mean ± SD. n = 4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001. (c) Diagram of the possible mechanism by which static magnetic field accelerates orthodontic tooth movement.

Article Snippet: Primary antibodies against rat CTSK (Proteintech, 11239‐1‐AP, 1:200), TNF‐α (Proteintech, 60291‐1‐Ig, 1:200) and IL‐1β (Proteintech, 26048‐1‐AP, 1:200) were used.

Techniques: Immunohistochemical staining, Staining

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs enhance orthodontic mechano‐transduction via activation in microtubule‐integrin assembly of PDLCs. (a) Heat map for cluster analysis of differentially expressed genes (DEGs). (b) Bubble plot of GO enrichment results of DEGs. (c) Bubble plot of KEGG enrichment results of DEGs. (d) Relative FPKM values of ITGB1, ITGB7, ITGB9, CCL7, TNF and IL6 from RNA‐seq data. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001. (e) 12h and 24h Relative gene expression of ITGB1, ITGB2, ITGB3, IFNG, MCSF and TNF from qRT‐PCR. Mean ± SD. n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001. (f) Representative immunofluorescence images of α‐Tubulin (green), Integrin‐β1 (red) (merged yellow) and nuclei (blue) in mechanical force applied PDLCs without and with SMFs. Scale bar: 10 µm. (g) Pearson's coefficient and Manders’ overlap coefficient (M1) for the colocalization analysis between α‐Tubulin and Integrin‐β1. Mean ± SD. n = 30. **** p < 0.0001.

Article Snippet: Primary antibodies against rat CTSK (Proteintech, 11239‐1‐AP, 1:200), TNF‐α (Proteintech, 60291‐1‐Ig, 1:200) and IL‐1β (Proteintech, 26048‐1‐AP, 1:200) were used.

Techniques: Transduction, Activation Assay, Analysis, RNA Sequencing, Gene Expression, Quantitative RT-PCR, Immunofluorescence

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet:

Article Snippet: Primary antibodies against rat CTSK (Proteintech, 11239‐1‐AP, 1:200), TNF‐α (Proteintech, 60291‐1‐Ig, 1:200) and IL‐1β (Proteintech, 26048‐1‐AP, 1:200) were used.

Techniques: Sequencing

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs amplify the response of orthodontic mechanical signaling pathways. (a) Representative immunofluorescence images of vinculin (green), F‐actin (purple), and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), with mechanical force only, and with mechanical force plus SMFs. Scale bar: 10 µm. (b) Cell area, (c) FA area per cell, and (d) cell shape index in PDLCs. Mean ± SD. n = 24. * p < 0.05, ** p < 0.01, **** p < 0.0001. (e) FAK: Focal Adhesion Kinase. It is a mechanotransducer that becomes activated upon orthodontic force, regulating downstream bone remodeling and inflammatory responses in PDLCs. Representative immunofluorescence images of FAK (red) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (f) Relative FAK integrated density per cell in PDLCs. Mean ± SD. n = 24. **** p < 0.0001. (g) Representative immunofluorescence images of YAP/TAZ (green) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (h) The nuclear‐cytoplasmic ratios of YAP/TAZ in PDLCs analyzed from the immunofluorescence images. Mean ± SD. n = 24. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

Techniques: Protein-Protein interactions, Immunofluorescence, Control

Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: SMFs amplify the response of orthodontic mechanical signaling pathways. (a) Representative immunofluorescence images of vinculin (green), F‐actin (purple), and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), with mechanical force only, and with mechanical force plus SMFs. Scale bar: 10 µm. (b) Cell area, (c) FA area per cell, and (d) cell shape index in PDLCs. Mean ± SD. n = 24. * p < 0.05, ** p < 0.01, **** p < 0.0001. (e) FAK: Focal Adhesion Kinase. It is a mechanotransducer that becomes activated upon orthodontic force, regulating downstream bone remodeling and inflammatory responses in PDLCs. Representative immunofluorescence images of FAK (red) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (f) Relative FAK integrated density per cell in PDLCs. Mean ± SD. n = 24. **** p < 0.0001. (g) Representative immunofluorescence images of YAP/TAZ (green) and nuclei (blue) in PDLCs under three conditions: control (without mechanical force and without SMFs), mechanical force only, and mechanical force plus SMFs. Scale bar: 10 µm. (h) The nuclear‐cytoplasmic ratios of YAP/TAZ in PDLCs analyzed from the immunofluorescence images. Mean ± SD. n = 24. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: PDLCs were respectively incubated with the primary antibody for Vinculin (Proteintech, 26520‐1‐Ap, 1:300), FAK (Proteintech, 12636‐1‐AP, 1:400), YAP/TAZ (Proteintech, 13584‐1‐Ap, 1:200), α‐Tubulin (Abclonal, AC012, 1:200) and Integrin‐β1 (Abclonal, A23497, 1:200).

Techniques: Protein-Protein interactions, Immunofluorescence, Control

Journal: MedComm

Article Title: Both Genetic Manipulation and Acupuncture Alleviate Depressive‐Like Behavior Mediated by Astrocytic P2X7 Receptors

doi: 10.1002/mco2.71042

Figure Lengend Snippet: Treatment with EA prevents the CUMS‐induced increase in expression of P2X7Rs. Determination by quantitative (q)PCR or Western blot of mRNA and protein for P2X7Rs, the purine‐metabolizing enzymes CD39 and CD73, the exocytotic vesicular proteins SNAP23 and SNAP25, and the ATP cellular release protein CALHM2 in mPFC brain slices of mice. p2x7, cd39, cd73, snap23, snap25, calhm2, mRNAs for the respective proteins. Determinations were under control conditions in the medial prefrontal cortex (mPFC) prepared from control mice, as well as in the mPFC of mice treated with CUMS either alone or in combination with EA or sham EA. (A–F) Expression of mRNAs as measured by the ΔΔCt method with respect to β‐actin‐RNA. Relative amounts of p2x7r (A), cd39 (B), cd73 (C), snap23 (D), snap25 (E), and calhm2 (F) mRNAs. (G–L) Representative immunoblots and quantification of the proteins for P2X7R (G), CD39 (H), CD73 (I), SNAP23 (J), SNAP25 (K), and CALHM2 (L) as a ratio of the spot of glycerinaldehyd‐3‐phosphat‐dehydrogenase (GAPDH). (M) Expression of p2x7R mRNA in control mice after EA or sham EA1 (see Section ) treatment. (N) Expression of P2X7 protein in control mice after EA or sham EA1 treatment. All data are shown as mean ± SEM. One‐way ANOVA was followed by the Holm–Sidak test for statistical analysis in case of normal distribution (A–L); * p < 0.05, ** p < 0.01 compared to the control group. The number of individual animals is indicated in each column.

Article Snippet: Membranes were blocked with 5% skimmed milk in Tris‐buffered saline with Tween 20 for 1 h at room temperature and subsequently incubated overnight at 4°C with the following primary antibodies: P2X7R (1:1000; Abcam, Cambridge, UK), CD39 (1:1000; Abcam), CD73 (1:1000; Proteintech, Rosemont, USA), SNAP23 (1:1000; Proteintech), SNAP25 (1:1000; Abcam), CALHM2 (1:400; Proteintech), and GAPDH (1:1000; Proteintech).

Techniques: Expressing, Western Blot, Control, Analysis